One of the most critical steps in multiplexed antibody panel design is selecting the right antibodies for your study. Choose the wrong antibody and you may lose weeks if not months, worth of work. An rigorous antibody validation strategy keeps your program on track by: Proving antibody specificity (the antibody’s ability to differentiate between various antigens), Proving affinity (antibody-epitope binding intensity)…
Organoids are tissue-derived adult stem cells that can be grow into self-organizing organotypic structures. Due to their derivation from human tissue, organoids can more accurately model the interactions between healthy human tissue and cancer, enabling patient-specific drug testing and development of tailored therapeutic regimens. The CODEX platform can image organoids utilizing a straightforward sample preparation and the standardized CODEX experimental protocol…
Seurat is an R package developed by Rahul Satija’s lab at the New York Genome Center. A widely used, open-source tool for single-cell analysis, Seurat was designed to explore single-cell RNA sequencing data. Seurat can also be applied to multiplex imaging-based spatial phenotyping data generated with CODEX®. Bioinformatics Field Application Scientist, Grady Carlson, PhD, shared the steps for using Seurat to analyze CODEX datasets…
The tumor microenvironment is undeniably complex. Specialized immune cells interact directly and indirectly with tumor cells in a constantly evolving relationship that influences the biology of the tumor. Spatial context is essential to uncovering the mysteries of cell behavior and interactions. Just as individuals may behave differently in cities, suburbs, and rural areas, individual cells adopt different roles depending on their surroundings, or neighborhoods. A pioneering study published in Cell by Dr. Garry Nolan’s lab at Stanford University introduced a novel analysis framework…
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